| Player | Player # | Position | Height | Weight | School |
| Branden Albert | 76 | T | 6'5 | 316 | Virginia |
| Charlie Anderson | 52 | LB | 6'4 | 250 | Mississippi |
| Javier Arenas | 21 | CB | 5'9 | 197 | Alabama |
| Jon Asamoah | 73 | G | 6'4 | 305 | Illinois |
| Harold Ayodele | 79 | DT | 6'1 | 339 | Tennessee State |
| Allen Bailey | 97 | DE | 6'3 | 288 | Miami (FL) |
| Brandon Bair | 90 | DE | 6'7 | 272 | Oregon |
| Mikail Baker | 41 | CB | 6'0 | 205 | Baylor |
| Jonathan Baldwin | 89 | WR | 6'4 | 230 | Pittsburgh |
| Shane Bannon | 40 | RB | 6'3 | 267 | Yale |
| Jackie Bates | 41 | CB | 5'10 | 180 | Hampton |
| Jackie Battle | 26 | RB | 6'2 | 238 | Houston |
| Anthony Becht | 88 | TE | 6'6 | 270 | West Virginia |
| Jovan Belcher | 59 | LB | 6'2 | 228 | Maine |
| Eric Berry | 29 | S | 6'0 | 211 | Tennessee |
| Kevin Boss | 0 | TE | 6'6 | 255 | Western Oregon |
| Dwayne Bowe | 82 | WR | 6'2 | 221 | LSU |
| Steve Breaston | 15 | WR | 6'0 | 189 | Michigan |
| Jalil Brown | 30 | CB | 6'1 | 204 | Colorado |
| Rob Bruggeman | 60 | C | 6'4 | 293 | Iowa |
| Caleb Campbell | 57 | LB | 6'2 | 237 | Army |
| Todd Carter | 14 | K | 6'1 | 190 | Grand Valley State |
| Matt Cassel | 7 | QB | 6'4 | 230 | USC |
| Tim Castille | 46 | RB | 5'11 | 238 | Alabama |
| Chris Chambers | 84 | WR | 5'11 | 210 | Wisconsin |
| Jamaal Charles | 25 | RB | 5'11 | 199 | Texas |
| Keary Colbert | 84 | WR | 6'1 | 205 | USC |
| Dustin Colquitt | 2 | P | 6'3 | 210 | Tennessee |
| Terrance Copper | 10 | WR | 6'0 | 207 | East Carolina |
| Brad Cottam | 87 | TE | 6'7 | 269 | Tennessee |
| Travis Daniels | 34 | CB | 6'1 | 195 | LSU |
| Glenn Dorsey | 72 | DE | 6'1 | 297 | LSU |
| Shaun Draughn | 32 | RB | 6'0 | 205 | North Carolina |
| Brandon Flowers | 24 | CB | 5'9 | 187 | Virginia Tech |
| Thomas Gafford | 43 | C | 6'2 | 250 | Houston |
| Dion Gales | 70 | DE | 6'5 | 310 | Troy |
| Charlie Gantt | 88 | TE | 6'4 | 260 | Michigan State |
| Wallace Gilberry | 92 | DE | 6'2 | 268 | Alabama |
| Amon Gordon | 99 | DT | 6'2 | 305 | Stanford |
| Bobby Greenwood | 74 | T | 6'5 | 278 | Alabama |
| Cory Greenwood | 93 | LB | 6'2 | 235 | Concordia (OR) |
| Kelly Gregg | 77 | DT | 6'0 | 320 | Oklahoma |
| Tamba Hali | 91 | LB | 6'3 | 275 | Penn State |
| Chris Harr | 60 | T | 6'6 | 260 | Chattanooga |
| Darryl Harris | 66 | G | 6'4 | 300 | Mississippi |
| Peyton Hillis | 0 | RB | 6'2 | 250 | Arkansas |
| Jeremy Horne | 11 | WR | 6'2 | 193 | Massachusetts |
| Justin Houston | 50 | LB | 6'3 | 258 | Georgia |
| Rodney Hudson | 61 | C | 6'2 | 299 | Florida State |
| Tyson Jackson | 94 | DE | 6'4 | 296 | LSU |
| Derrick Johnson | 56 | LB | 6'3 | 242 | Texas |
| Tervaris Johnson | 38 | RB | 6'2 | 248 | Miami (FL) |
| Thomas Jones | 20 | RB | 5'10 | 212 | Virginia |
| Amara Kamara | 60 | LB | 6'1 | 240 | Temple |
| Reshard Langford | 48 | S | 6'1 | 213 | Vanderbilt |
| Javes Lewis | 41 | S | 6'1 | 190 | Oregon |
| Kendrick Lewis | 23 | S | 6'0 | 198 | Mississippi |
| Ryan Lilja | 65 | G | 6'2 | 290 | Kansas State |
| Steve Maneri | 87 | TE | 6'6 | 290 | Temple |
| Zeke Markshausen | 5 | WR | 5'11 | 185 | Northwestern |
| Corey Mays | 51 | LB | 6'1 | 245 | Notre Dame |
| Kyle McCarthy | 41 | S | 6'1 | 210 | Notre Dame |
| Dexter McCluster | 22 | RB | 5'8 | 170 | Mississippi |
| Jon McGraw | 47 | S | 6'3 | 208 | Kansas State |
| Gabe Miller | 51 | LB | 6'3 | 257 | Oregon State |
| David Mims | 70 | T | 6'8 | 335 | Virginia Union |
| Tony Moeaki | 81 | TE | 6'3 | 252 | Iowa |
| Jamar Newsome | 84 | WR | 6'1 | 201 | UCF |
| Ryan O'Callaghan | 75 | T | 6'7 | 330 | California |
| Jake O'Connell | 85 | TE | 6'3 | 250 | Miami (OH) |
| Tyler Palko | 4 | QB | 6'1 | 215 | Pittsburgh |
| Lucas Patterson | 74 | G | 6'4 | 295 | Texas A&M |
| Josue Paul | 9 | WR | 6'1 | 190 | Central Connecticut State |
| Sabby Piscitelli | 42 | S | 6'3 | 224 | Oregon State |
| Jerrell Powe | 95 | NT | 6'2 | 331 | Mississippi |
| Ricky Price | 35 | S | 6'1 | 195 | Oklahoma State |
| Brady Quinn | 0 | QB | 6'3 | 235 | Notre Dame |
| Jacques Reeves | 0 | CB | 5'11 | 188 | Purdue |
| Stanford Routt | 0 | CB | 6'1 | 195 | Houston |
| Mario Russell | 38 | S | 6'2 | 205 | Carson-Newman |
| Cameron Sheffield | 55 | LB | 6'2 | 257 | Troy |
| Brandon Siler | 52 | LB | 6'2 | 239 | Florida |
| Cody Slate | 80 | TE | 6'4 | 229 | Marshall |
| Ricky Stanzi | 13 | QB | 6'4 | 228 | Iowa |
| Andy Studebaker | 96 | LB | 6'3 | 248 | Wheaton (IL) |
| Ryan Succop | 6 | K | 6'2 | 218 | South Carolina |
| Anthony Toribio | 98 | DT | 6'1 | 315 | Carson-Newman |
| Verran Tucker | 15 | WR | 6'1 | 204 | California |
| Jerheme Urban | 83 | WR | 6'3 | 207 | Trinity (TX) |
| Mike Vrabel | 50 | LB | 6'4 | 261 | Ohio State |
| Pierre Walters | 97 | LB | 6'5 | 269 | Eastern Illinois |
| Demond Washington | 32 | S | 5'9 | 182 | Auburn |
| Donald Washington | 27 | S | 6'1 | 197 | Ohio State |
| Casey Wiegmann | 62 | C | 6'2 | 285 | Iowa |
| Chandler Williams | 8 | WR | 5'11 | 176 | Florida International |
| Eric Winston | 0 | T | 6'7 | 302 | Miami (FL) |
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