| Player | Player # | Position | Height | Weight | School |
| Flozell Adams | 71 | T | 6'7 | 338 | Michigan State |
| Cortez Allen | 28 | CB | 6'1 | 196 | Citadel |
| Will Allen | 26 | S | 6'1 | 200 | Ohio State |
| Baron Batch | 35 | RB | 5'10 | 210 | Texas Tech |
| Charlie Batch | 16 | QB | 6'2 | 216 | Eastern Michigan |
| Aaron Bates | 3 | P | 6'0 | 198 | Michigan State |
| Arnaz Battle | 81 | WR | 6'1 | 208 | Notre Dame |
| Tyler Beiler | 19 | WR | 6'0 | 190 | Bridgewater (VA) |
| Mike Blanc | 66 | DE | 6'4 | 289 | Auburn |
| Ty Boyle | 61 | DE | 6'4 | 286 | North Dakota |
| Eugene Bright | 87 | TE | 6'4 | 268 | Purdue |
| Niles Brinkley | 27 | CB | 5'10 | 190 | Wisconsin |
| Dorian Brooks | 77 | G | 6'2 | 306 | James Madison |
| Antonio Brown | 84 | WR | 5'10 | 186 | Central Michigan |
| Curtis Brown | 31 | CB | 6'0 | 185 | Texas |
| Corbin Bryant | 95 | DE | 6'4 | 300 | Northwestern |
| Chris Carter | 54 | LB | 6'1 | 248 | Fresno State |
| Vaughn Charlton | 49 | TE | 6'4 | 252 | Temple |
| Miguel Chavis | 65 | DE | 6'5 | 285 | Clemson |
| Erik Clanton | 38 | LB | 6'3 | 235 | Citadel |
| Ryan Clark | 25 | S | 5'11 | 205 | LSU |
| John Clay | 38 | RB | 6'1 | 248 | Wisconsin |
| Willie Colon | 74 | T | 6'3 | 315 | Hofstra |
| Jerricho Cotchery | 89 | WR | 6'1 | 200 | North Carolina State |
| Jarrett Crittenton | 61 | DE | 6'6 | 290 | Middle Tennessee State |
| Da'Mon Cromartie-Smith | 42 | S | 6'2 | 210 | UTEP |
| Chris Dieker | 2 | QB | 6'5 | 235 | Southern Illinois |
| Dennis Dixon | 10 | QB | 6'3 | 209 | Oregon |
| Kevin Dockery | 37 | CB | 5'8 | 188 | Mississippi State |
| Jonathan Dwyer | 27 | RB | 5'11 | 229 | Georgia Tech |
| Chris Ellis | 90 | LB | 6'4 | 267 | Virginia Tech |
| Trai Essex | 79 | G | 6'5 | 324 | Northwestern |
| James Farrior | 51 | LB | 6'2 | 243 | Virginia |
| Larry Foote | 50 | LB | 6'1 | 239 | Michigan |
| Ramon Foster | 73 | G | 6'6 | 325 | Tennessee |
| Marcus Gilbert | 77 | T | 6'6 | 330 | Florida |
| David Gilreath | 18 | WR | 5'11 | 169 | Wisconsin |
| Anthony Gray | 67 | NT | 6'0 | 317 | Southern Miss |
| Brett Greenwood | 35 | S | 5'11 | 198 | Iowa |
| Eric Greenwood | 8 | WR | 6'7 | 223 | Idaho |
| Casey Hampton | 98 | NT | 6'1 | 325 | Texas |
| Macho Harris | 31 | S | 6'0 | 200 | Virginia Tech |
| Tuff Harris | 37 | S | 6'0 | 198 | Montana |
| James Harrison | 92 | LB | 6'0 | 242 | Kent State |
| Josh Harrison | 57 | T | 6'4 | 310 | South Carolina State |
| Cameron Heyward | 97 | DE | 6'5 | 288 | Ohio State |
| Brandon Hicks | 44 | LB | 6'2 | 232 | Florida |
| Chris Hoke | 76 | NT | 6'2 | 305 | Brigham Young |
| Ziggy Hood | 96 | DE | 6'3 | 300 | Missouri |
| Mortty Ivy | 57 | LB | 6'1 | 239 | West Virginia |
| David Johnson | 85 | TE | 6'2 | 260 | Arkansas State |
| James Johnson | 41 | RB | 5'11 | 197 | Kansas State |
| Jerrod Johnson | 8 | QB | 6'5 | 251 | Texas A&M |
| Will Johnson | 0 | RB | 6'2 | 240 | West Virginia |
| Eli Joseph | 65 | DE | 6'3 | 275 | Temple |
| Jeremy Kapinos | 13 | P | 6'1 | 233 | Penn State |
| Brett Keisel | 99 | DE | 6'5 | 285 | Brigham Young |
| Chris Kemoeatu | 68 | G | 6'3 | 344 | Utah |
| Byron Leftwich | 4 | QB | 6'5 | 250 | Marshall |
| Doug Legursky | 64 | G | 6'1 | 315 | Marshall |
| Keenan Lewis | 23 | CB | 6'0 | 208 | Oregon State |
| Wes Lyons | 15 | TE | 6'8 | 233 | West Virginia |
| Anthony Madison | 37 | CB | 5'9 | 180 | Alabama |
| John Malecki | 62 | G | 6'2 | 298 | Pittsburgh |
| Chris McCoy | 46 | LB | 6'3 | 261 | Middle Tennessee State |
| Jamie McCoy | 45 | TE | 6'3 | 240 | Texas A&M |
| Terrence McCrae | 11 | WR | 6'3 | 194 | Ohio |
| Bryant McFadden | 20 | CB | 6'0 | 190 | Florida State |
| Marshall McFadden | 40 | LB | 6'2 | 235 | South Carolina State |
| Walter McFadden | 39 | CB | 5'10 | 180 | Auburn |
| Steve McLendon | 90 | NT | 6'4 | 280 | Troy |
| Rashard Mendenhall | 34 | RB | 5'10 | 225 | Illinois |
| Heath Miller | 83 | TE | 6'5 | 256 | Virginia |
| Adam Mims | 18 | WR | 5'9 | 193 | Furman |
| Kenneth Moore | 18 | WR | 5'11 | 195 | Wake Forest |
| Mewelde Moore | 21 | RB | 5'11 | 209 | Tulane |
| Ryan Mundy | 29 | S | 6'1 | 209 | West Virginia |
| Troy Polamalu | 43 | S | 5'10 | 207 | USC |
| Leonard Pope | 0 | TE | 6'8 | 264 | Georgia |
| Maurkice Pouncey | 53 | C | 6'4 | 304 | Florida |
| Antwaan Randle El | 82 | WR | 5'10 | 185 | Indiana |
| Isaac Redman | 33 | RB | 6'0 | 230 | Bowie State |
| Armand Robinson | 14 | WR | 6'1 | 199 | Miami (OH) |
| Ben Roethlisberger | 7 | QB | 6'5 | 241 | Miami (OH) |
| Myron Rolle | 45 | S | 6'2 | 219 | Florida State |
| Emmanuel Sanders | 88 | WR | 5'11 | 180 | Southern Methodist |
| Weslye Saunders | 82 | TE | 6'5 | 270 | South Carolina |
| Chris Scott | 71 | T | 6'4 | 319 | Tennessee |
| Jonathan Scott | 72 | T | 6'6 | 318 | Texas |
| Daniel Sepulveda | 9 | P | 6'3 | 230 | Baylor |
| Aaron Smith | 91 | DE | 6'5 | 298 | Northern Colorado |
| Antonio Smith | 41 | CB | 5'9 | 190 | Ohio State |
| Troy Smith | 5 | QB | 6'0 | 217 | Ohio State |
| Chad Spann | 30 | RB | 5'9 | 198 | Northern Illinois |
| Max Starks | 78 | T | 6'8 | 345 | Florida |
| Juamorris Stewart | 87 | WR | 6'3 | 205 | Southern University |
| Shaun Suisham | 6 | K | 6'0 | 200 | Bowling Green |
| Limas Sweed | 80 | WR | 6'4 | 220 | Texas |
| Stevenson Sylvester | 55 | LB | 6'2 | 231 | Utah |
| Ike Taylor | 24 | CB | 6'2 | 195 | Louisiana-Lafayette |
| Lawrence Timmons | 94 | LB | 6'1 | 234 | Florida State |
| Trevis Turner | 67 | T | 6'7 | 330 | Abilene Christian |
| Mike Wallace | 17 | WR | 6'0 | 199 | Mississippi |
| Hines Ward | 86 | WR | 6'0 | 205 | Georgia |
| Greg Warren | 60 | C | 6'3 | 252 | North Carolina |
| Swayze Waters | 5 | K | 5'11 | 181 | UAB |
| Kade Weston | 67 | DT | 6'5 | 315 | Georgia |
| Derrick Williams | 15 | WR | 5'11 | 197 | Penn State |
| LaMarr Woodley | 56 | LB | 6'2 | 265 | Michigan |
| Al Woods | 65 | DE | 6'4 | 307 | LSU |
| Jason Worilds | 93 | LB | 6'2 | 262 | Virginia Tech |
| Albert Young | 41 | RB | 5'10 | 209 | Iowa |
| Jimmy Young | 14 | WR | 6'1 | 204 | TCU |
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