| Player | Player # | Position | Height | Weight | School |
| David Akers | 2 | K | 5'10 | 200 | Louisville |
| Chase Beeler | 61 | C | 6'3 | 285 | Stanford |
| Chris Blohm | 0 | TE | 6'4 | 262 | Yale |
| Dontavia Bogan | 9 | WR | 6'1 | 188 | South Florida |
| Alex Boone | 75 | T | 6'8 | 300 | Ohio State |
| Navorro Bowman | 53 | LB | 6'0 | 242 | Penn State |
| Tramaine Brock | 26 | CB | 5'10 | 197 | Belhaven College |
| Ahmad Brooks | 55 | LB | 6'3 | 259 | Virginia |
| Tarell Brown | 25 | CB | 5'10 | 193 | Texas |
| Brian Bulcke | 71 | DE | 6'4 | 285 | Stanford |
| Nate Byham | 82 | TE | 6'4 | 264 | Pittsburgh |
| Rock Cartwright | 28 | RB | 5'8 | 215 | Kansas State |
| Garrett Chisolm | 0 | G | 6'6 | 299 | South Carolina |
| Jack Corcoran | 43 | RB | 6'1 | 234 | Rutgers |
| Perrish Cox | 20 | CB | 6'0 | 198 | Oklahoma State |
| Michael Crabtree | 15 | WR | 6'1 | 214 | Texas Tech |
| Chris Culliver | 29 | CB | 6'0 | 199 | South Carolina |
| Anthony Davis | 76 | T | 6'5 | 323 | Rutgers |
| Bruce Davis | 58 | LB | 6'3 | 250 | UCLA |
| Phillip Davis | 33 | CB | 5'10 | 173 | Tulane |
| Vernon Davis | 85 | TE | 6'3 | 250 | Maryland |
| Anthony Dixon | 24 | RB | 6'1 | 233 | Mississippi State |
| Demarcus Dobbs | 96 | DT | 6'6 | 275 | Georgia |
| Braylon Edwards | 17 | WR | 6'3 | 214 | Michigan |
| Donovan Edwards | 63 | C | 6'5 | 280 | California |
| Demetric Evans | 93 | DE | 6'4 | 275 | Georgia |
| Ted Ginn Jr. | 19 | WR | 5'11 | 180 | Ohio State |
| Dashon Goldson | 38 | S | 6'2 | 200 | Washington |
| Tavares Gooden | 56 | LB | 6'1 | 242 | Miami (FL) |
| Jonathan Goodwin | 59 | C | 6'3 | 318 | Michigan |
| Frank Gore | 21 | RB | 5'9 | 217 | Miami (FL) |
| Larry Grant | 54 | LB | 6'1 | 251 | Ohio State |
| Derek Hall | 71 | T | 6'5 | 307 | Stanford |
| Parys Haralson | 98 | LB | 6'0 | 255 | Tennessee |
| Joe Hastings | 13 | WR | 6'0 | 185 | Washburn |
| Eric Heitmann | 66 | C | 6'3 | 312 | Stanford |
| Curtis Holcomb | 41 | CB | 5'10 | 190 | Florida A&M |
| Kendall Hunter | 32 | RB | 5'7 | 199 | Oklahoma State |
| Mike Iupati | 77 | G | 6'5 | 331 | Idaho |
| Brandon Jacobs | 0 | RB | 6'4 | 264 | Southern Illinois |
| William James | 31 | CB | 6'0 | 200 | Western Illinois |
| Ricky Jean Francois | 95 | DT | 6'3 | 295 | LSU |
| Brian Jennings | 86 | TE | 6'5 | 242 | Arizona State |
| Josh Johnson | 0 | QB | 6'3 | 205 | San Diego |
| Colin Jones | 43 | S | 6'0 | 208 | TCU |
| Alex Joseph | 58 | LB | 6'2 | 240 | Temple |
| Colin Kaepernick | 7 | QB | 6'4 | 230 | Nevada |
| Daniel Kilgore | 67 | G | 6'3 | 308 | Appalachian State |
| Keaton Kristick | 57 | LB | 6'3 | 226 | Oregon State |
| Nate Lawrie | 83 | TE | 6'6 | 255 | Yale |
| Andy Lee | 4 | P | 6'2 | 180 | Pittsburgh |
| Mark LeGree | 0 | S | 6'0 | 210 | Appalachian State |
| Mario Manningham | 0 | WR | 6'0 | 185 | Michigan |
| Jeremiah Masoli | 3 | QB | 5'11 | 220 | Mississippi |
| John Matthews | 83 | WR | 6'0 | 200 | San Diego |
| Ray McDonald | 91 | DT | 6'3 | 290 | Florida |
| Bruce Miller | 49 | RB | 6'2 | 248 | UCF |
| Randy Moss | 84 | WR | 6'4 | 210 | Marshall |
| Joe Nedney | 6 | K | 6'5 | 234 | San Jose State |
| Cory Nelms | 23 | CB | 6'0 | 195 | Miami (FL) |
| Kyle Nelson | 0 | TE | 6'2 | 245 | New Mexico State |
| Moran Norris | 44 | RB | 6'1 | 250 | Kansas |
| Chris Patrick | 61 | T | 6'4 | 280 | Nebraska |
| Sam Paulescu | 3 | P | 6'0 | 189 | Oregon State |
| Justin Peelle | 81 | TE | 6'4 | 251 | Oregon |
| Mike Person | 78 | G | 6'4 | 299 | Montana State |
| Ryan Pontbriand | 0 | C | 6'2 | 255 | Rice |
| Konrad Reuland | 88 | TE | 6'4 | 260 | Stanford |
| Carlos Rogers | 22 | CB | 6'0 | 192 | Auburn |
| Kenny Rowe | 50 | LB | 6'3 | 232 | Oregon |
| Fabrizio Scaccia | 9 | K | 6'1 | 210 | Indian River CC |
| Barry Sims | 65 | T | 6'5 | 300 | Utah |
| Aldon Smith | 99 | LB | 6'4 | 258 | Missouri |
| Alex Smith | 11 | QB | 6'4 | 217 | Utah |
| Justin Smith | 94 | DT | 6'4 | 285 | Missouri |
| Seth Smith | 26 | RB | 5'8 | 198 | New Mexico State |
| Isaac Sopoaga | 90 | DT | 6'2 | 330 | Hawaii |
| C.J. Spillman | 27 | S | 6'0 | 199 | Marshall |
| Joe Staley | 74 | T | 6'5 | 315 | Central Michigan |
| Brett Swain | 18 | WR | 6'0 | 200 | San Diego State |
| Scott Tolzien | 3 | QB | 6'3 | 208 | Wisconsin |
| Will Tukuafu | 92 | DT | 6'4 | 293 | Oregon |
| Delanie Walker | 46 | TE | 6'0 | 242 | Central Missouri |
| Anthony West | 45 | CB | 6'0 | 205 | Nebraska |
| Brian Westbrook | 20 | RB | 5'10 | 203 | Villanova |
| Donte Whitner | 31 | S | 5'10 | 208 | Ohio State |
| Kenny Wiggins | 0 | T | 6'6 | 314 | Fresno State |
| Michael Wilhoite | 57 | LB | 6'0 | 240 | Washburn |
| Ian Williams | 93 | NT | 6'1 | 305 | Notre Dame |
| Kyle Williams | 10 | WR | 5'10 | 186 | Arizona State |
| Patrick Willis | 52 | LB | 6'1 | 240 | Mississippi |
| Dominique Zeigler | 17 | WR | 6'2 | 181 | Baylor |
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