| Player | Player # | Position | Height | Weight | School |
| Phillip Adams | 40 | CB | 5'11 | 192 | South Carolina State |
| Ladi Ajiboye | 97 | DT | 6'2 | 278 | South Carolina |
| Jay Alford | 69 | DT | 6'3 | 305 | Penn State |
| Pierre Allen | 95 | DE | 6'5 | 265 | Nebraska |
| Doug Baldwin | 89 | WR | 5'10 | 189 | Stanford |
| Tressor Baptiste | 0 | LB | 6'0 | 240 | Texas A&M-Kingsville |
| Allen Barbre | 78 | T | 6'4 | 305 | Missouri Southern State |
| Dorson Boyce | 43 | RB | 6'2 | 231 | Washington |
| Allen Bradford | 44 | LB | 5'11 | 235 | USC |
| Alan Branch | 99 | DT | 6'6 | 325 | Michigan |
| James Brindley | 37 | S | 5'11 | 189 | Utah State |
| Raheem Brock | 98 | DE | 6'4 | 274 | Temple |
| Brandon Browner | 39 | CB | 6'4 | 221 | Oregon State |
| Red Bryant | 79 | DE | 6'4 | 323 | Texas A&M |
| Deon Butler | 11 | WR | 5'10 | 182 | Penn State |
| Wes Byrum | 5 | K | 6'2 | 211 | Auburn |
| James Carpenter | 75 | T | 6'5 | 321 | Alabama |
| Chris Carter | 16 | WR | 6'0 | 195 | UC Davis |
| Kam Chancellor | 31 | S | 6'3 | 232 | Virginia Tech |
| Chris Clemons | 91 | DE | 6'3 | 254 | Georgia |
| Colin Cole | 90 | DT | 6'2 | 328 | Iowa |
| Kennard Cox | 35 | CB | 6'0 | 191 | Pittsburgh |
| Keith Darbut | 48 | LB | 6'5 | 235 | Baldwin-Wallace |
| Dexter Davis | 58 | DE | 6'1 | 244 | Arizona State |
| Kris Durham | 16 | WR | 6'6 | 216 | Georgia |
| Paul Fanaika | 66 | G | 6'5 | 327 | Arizona State |
| Heath Farwell | 55 | LB | 6'0 | 235 | San Diego State |
| Jay Finley | 33 | RB | 5'11 | 202 | Baylor |
| Matt Flynn | 15 | QB | 6'2 | 225 | LSU |
| Justin Forsett | 20 | RB | 5'8 | 198 | California |
| Coye Francies | 22 | CB | 6'0 | 185 | San Jose State |
| Breno Giacomini | 68 | T | 6'7 | 318 | Louisville |
| John Gold | 1 | P | 6'3 | 205 | Texas |
| John Graves | 90 | DE | 6'3 | 286 | Virginia Tech |
| Clint Gresham | 49 | C | 6'3 | 240 | TCU |
| Steven Hauschka | 4 | K | 6'4 | 210 | North Carolina State |
| Chris Henry | 40 | RB | 5'11 | 233 | Arizona |
| Anthony Heygood | 53 | LB | 6'2 | 230 | Purdue |
| Leroy Hill | 56 | LB | 6'1 | 238 | Clemson |
| Jesse Hoffman | 44 | CB | 6'2 | 210 | Eastern Washington |
| Neal Howey | 42 | LB | 6'1 | 215 | Eastern Michigan |
| Tarvaris Jackson | 7 | QB | 6'2 | 225 | Alabama State |
| Edgerrin James | 32 | RB | 6'0 | 219 | Miami (FL) |
| Lemuel Jeanpierre | 61 | G | 6'3 | 301 | South Carolina |
| Jeron Johnson | 32 | S | 5'10 | 212 | Boise State |
| Michael Johnson | 54 | LB | 6'0 | 254 | North Alabama |
| Jason Jones | 0 | DE | 6'5 | 276 | Eastern Michigan |
| Jarriel King | 77 | T | 6'5 | 317 | South Carolina |
| Jameson Konz | 46 | LB | 6'3 | 234 | Kent State |
| Zac Lee | 5 | QB | 6'2 | 215 | Nebraska |
| Lazarius Levingston | 93 | DE | 6'4 | 292 | LSU |
| Roy Lewis | 34 | CB | 5'10 | 190 | Washington |
| Ricardo Lockette | 83 | WR | 6'2 | 211 | Fort Valley State |
| Kregg Lumpkin | 0 | RB | 5'11 | 228 | Georgia |
| Deuce Lutui | 0 | G | 6'4 | 338 | USC |
| Marshawn Lynch | 24 | RB | 5'11 | 215 | California |
| Chris Maragos | 42 | S | 5'10 | 200 | Wisconsin |
| Charly Martin | 0 | WR | 6'1 | 212 | West Texas A&M |
| Byron Maxwell | 41 | CB | 6'1 | 207 | Clemson |
| Anthony McCoy | 85 | TE | 6'5 | 259 | USC |
| Matt McCoy | 52 | LB | 6'0 | 232 | San Diego State |
| Clinton McDonald | 69 | DT | 6'2 | 297 | Memphis |
| Paul McQuistan | 67 | G | 6'6 | 315 | Weber State |
| Brandon Mebane | 92 | DT | 6'1 | 311 | California |
| Zach Miller | 86 | TE | 6'5 | 255 | Arizona State |
| Lawyer Milloy | 36 | S | 6'0 | 211 | Washington |
| Barrett Moen | 0 | DT | 6'3 | 275 | Minnesota |
| John Moffitt | 74 | G | 6'4 | 319 | Wisconsin |
| Michael Morgan | 48 | LB | 6'4 | 220 | USC |
| Cameron Morrah | 88 | TE | 6'3 | 251 | California |
| Adrian Moten | 59 | LB | 6'2 | 230 | Maryland |
| John Nalbone | 82 | TE | 6'4 | 255 | Monmouth |
| Ben Obomanu | 87 | WR | 6'1 | 204 | Auburn |
| Russell Okung | 76 | T | 6'5 | 310 | Oklahoma State |
| Frank Omiyale | 0 | T | 6'4 | 315 | Tennessee Tech |
| Brent Osborne | 62 | C | 6'5 | 285 | Harvard |
| Ron Parker | 36 | CB | 6'0 | 206 | Newberry |
| Josh Pinkard | 22 | CB | 6'1 | 218 | USC |
| Chester Pitts | 69 | G | 6'4 | 308 | San Diego State |
| Caz Piurowski | 69 | T | 6'7 | 271 | Florida State |
| Josh Portis | 2 | QB | 6'3 | 211 | California (PA) |
| Jeff Reed | 3 | K | 5'11 | 225 | North Carolina |
| Sidney Rice | 18 | WR | 6'4 | 202 | South Carolina |
| Michael Robinson | 26 | RB | 6'1 | 240 | Penn State |
| Barrett Ruud | 0 | LB | 6'2 | 241 | Nebraska |
| Jon Ryan | 9 | P | 6'0 | 217 | Regina |
| A.J. Schable | 63 | DE | 6'3 | 281 | South Dakota |
| Richard Sherman | 25 | CB | 6'3 | 195 | Stanford |
| Junior Siavii | 94 | DT | 6'5 | 315 | Oregon |
| Ryan Sims | 98 | DT | 6'4 | 315 | North Carolina |
| Brandon Smith | 3 | WR | 6'2 | 202 | Arizona State |
| Malcolm Smith | 53 | LB | 6'0 | 226 | USC |
| Blake Sorensen | 54 | LB | 6'1 | 227 | Wisconsin |
| Tyrell Sutton | 0 | RB | 5'8 | 213 | Northwestern |
| Golden Tate | 81 | WR | 5'10 | 202 | Notre Dame |
| Vai Taua | 47 | RB | 5'10 | 211 | Nevada |
| Adrian Taylor | 0 | DT | 6'4 | 291 | Oklahoma |
| Craig Terrill | 93 | DT | 6'2 | 296 | Purdue |
| Rickey Thenarse | 38 | S | 6'0 | 205 | Nebraska |
| Earl Thomas | 29 | S | 5'10 | 202 | Texas |
| Walter Thurmond | 28 | CB | 5'11 | 190 | Oregon |
| Ryan Travis | 48 | TE | 6'3 | 235 | West Liberty State |
| Marcus Trufant | 23 | CB | 5'11 | 197 | Washington State |
| Max Unger | 60 | C | 6'5 | 305 | Oregon |
| David Vobora | 54 | LB | 6'1 | 239 | Idaho |
| Derek Walker | 78 | DE | 6'4 | 270 | Illinois |
| Leon Washington | 33 | RB | 5'8 | 203 | Florida State |
| Teryl White | 62 | DT | 6'5 | 290 | North Carolina Central |
| Jimmy Wilkerson | 97 | DE | 6'2 | 270 | Oklahoma |
| Mike Williams | 17 | WR | 6'5 | 235 | USC |
| K.J. Wright | 50 | LB | 6'4 | 246 | Mississippi State |
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