Juventus coach Massimiliano Allegri is hopeful that Dybala will recover during the international break and that the Argentina forward will be fit to face Napoli in the league and then in the second leg of their Italian Cup semifinal, which it leads 3-1.
Dybala limped off shortly before the half-hour mark with a muscle problem and was replaced by Marko Pjaca.
"Paulo felt a twinge at the back of his thigh but shouldn’t be anything serious," Allegri said. "He is a player who has unique characteristics in linking up the lines, Pjaca doesn’t have this type of play among his skills."
Juventus hosts Barcelona in the first leg of their Champions League quarterfinal on April 11.
"Playing a quarterfinal against Barcelona has to become a norm for Juve," Allegri said. "You can go out or you can go through but the other teams have to respect us and I think that in the last few seasons they've begun to do so. "
Juventus remained on course for an unprecedented sixth successive Serie A title as it stayed eight points clear of Roma and 10 ahead of Napoli.
"It was an important step," Allegri said. "It was fundamental to win this match because we play Napoli after the break. We had one of the best first halves of the season, for our intensity and the chances we created."
Juan Cuadrado scored the only goal of the match in the seventh minute, heading in Kwadwo Asamoah's cross.
Reserve goalkeeper Christian Puggioni was in for the suspended Emiliano Viviano and he pulled off several fine saves to deny Gonzalo Higuain.
Juventus goalkeeper Gianluigi Buffon set a new club record when he passed 39,681 Serie A minutes for the Bianconeri.
"I'm happy to have reached this number," the 39-year-old Juventus and Italy captain said. "To have done so as part of such a strong team, winning so many trophies, is even more pleasing ... I hope to have many more crucial minutes in my legs, my head, my heart and my hands."
EMPOLI 2, NAPOLI 3
Napoli risked losing a three-goal lead but held on for its first win at the Stadio Castellani on coach Maurizio Sarri's return to his old club.
Dries Mertens, who had missed an early penalty, netted his 20th league goal this season and Lorenzo Insigne scored twice to give Napoli a commanding lead before Empoli hit back through former Napoli player Omar El Kaddouri and Massimo Maccarone.
"We are happy for the win, but you don't grow like this," Napoli captain Marek Hamsik said. "We can't give a whole half to our opponents and risk compromising everything we did well ... We won and that's good, but we have to talk. We want to get into the Champions League automatically."
The visitors were awarded a controversial penalty in the sixth minute when Mertens was fouled by Andrea Costa, although it was unclear whether the challenge was inside the box.
Mertens' spot kick was parried by Lukasz Skorupski.
Costa was at fault for Napoli's opener in the 19th as he headed Faouzi Ghoulam's cross to Insigne, who slotted home from close range.
Mertens atoned for his penalty miss five minutes later as he lifted a stunning free kick into the top right corner. It was his 18th goal in his past 15 matches.
Napoli was awarded another penalty eight minutes from halftime and this time Insigne stepped up to convert after Manuel Pasqual tripped Jose Callejon.
Empoli had only scored 15 goals all season but got one back in the 70th when El Kaddouri curled in a free kick.
Maccarone set up a tense finale when he converted a penalty eight minutes from time after Ghoulam tripped Rade Krunic. It was his 100th goal for the club.
ROMA 3, SASSUOLO 1
Edin Dzeko was again on target as Roma maintained its push for the second automatic Champions League spot.
Dzeko scored a brilliant third goal for Roma in the 68th, eight minutes after coming on as a substitute, following a one-two with Kevin Strootman.
It was Dzeko's 21st league goal of the season, putting him one behind league leader Andrea Belotti of Torino.
Mohamed Salah had given Roma the lead on the stroke of halftime after Leandro Paredes cancelled out Gregoire Defrel's early opener for Sassuolo.
Lazio's five-match winning run came to an end as it was held to a goalless draw at Cagliari to dent its hopes of a Champions League spot.
Lazio slipped six points behind Napoli.
Atalanta closed to within two points of Lazio with a 3-0 win over last-placed Pescara. Alejandro Gomez scored twice and set up the other for Alberto Grassi as Atalanta bounced back from last weekend's 7-1 defeat at Inter Milan.
Nikola Kalinic scored in the final minute for the second successive match as Fiorentina won 1-0 at Crotone, while Bologna beat Chievo Verona 4-1.
Udinese came from behind to beat 10-man Palermo 4-1.