Tatis fielded the ball, made the throw to first base and then began walking off the field. He was met by manager Jayce Tingler and a trainer. After a few minutes in the dugout, Tatis headed toward the clubhouse accompanied by a trainer.
The Padres said Tatis will be reevaluated on Wednesday.
Tatis signed the longest contract in baseball history on Feb. 22, a $340 million, 14-year deal.
Other than that, it hasn't been a smooth spring for the 22-year-old superstar. He missed time with flu-like symptoms and then jarred himself after diving headfirst into home on a flyball to shallow left field in a game on March 13.