The three-time All-Star leaped near the centre-field wall to catch a ball hit by Rays second baseman Brandon Lowe before landing awkwardly.
He was checked out by the training staff.
Springer finished the inning in the outfield before taking an at-bat in the top of the third and ultimately left the game afterward.
Raimel Tapia replaced Springer in the Blue Jays’ lineup.
Blue Jays manager Charlie Montoyo provided an update post-game and said Springers will be out day-to-day for now.
The Blue Jays fell 5-2 to the Rays as their losing streak extended to five games.
Hyu Jin Ryu takes the mound for the second game of the set Saturday with the first pitch scheduled for 6:10 p.m. ET. Coverage begins with Blue Jays Central at 5:30 p.m. ET/ 2:30 p.m. PT on Sportsnet ONE and Sportsnet NOW.