Barkov is expected to return after sitting out since March 23 with a lower-body injury. Hornqvist will also return for the first time since getting injured against the Chicago Blackhawks on March 26.
At the time of injury, Barkov led the Panthers with 37 points through 31 games, but now sits behind Jonathan Huberdeau who has 42 points in 37 games. Hornqvist has 25 points through 33 games this season.
The Panthers will take on the Columbus Blue Jackets, with Florida winning three of the last four meetings between the two teams.