Stamkos got tangled up with Blackhawks forward Joey Anderson late in the first period. As Stamkos got up from the ice, he appeared to tweak something in his left leg and was slow to get to the bench.
He headed down the tunnel, straight to the locker room and did not return to the game.
“I think he’s going to be OK,” Cooper told reporters after the game. “We just kept him out for precaution. We’ll check him out tomorrow, but we’re hopeful he’ll be OK.”
The 33-year-old Stamkos has 27 goals and 66 points in 65 games this season for the Lightning.
The Lightning now hold a 39-21-6 record and are four points back of the Toronto Maple Leafs for second place in the Atlantic Division.