Canadian Leylah Annie Fernandez lost her second-round match 6-2, 6-4 to Barbora Krejcikova of the Czech Republic.
Krejcikova, the 11th ranked player in the world, won the 2021 French Open in both singles and doubles.
Felix Auger-Aliassime and Gabriela Dabrowski are the only Canadians in Tokyo as the duo will compete in the mixed doubles tournament.