The Penguins play their next two games on the road against Tampa Bay and Florida, before hosting the Anaheim Ducks on Monday.
Malkin leads the Penguins in goals (23) and points (49), and is second on the team in assists with 26, having played in all 49 games this year.
Eric Fehr also missed practice Thursday, where it was announced that the 30-year-old forward would miss a month with a "significant lower-body injury."
Fehr missed the first 10 games of the season after undergoing surgery on his elbow. He's played in every game since, tallying two goals and five assists.